Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 6589..7124 Replicon plasmid A
Accession NZ_CP010214
Organism Escherichia coli strain M15

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag RG61_RS26620 Protein ID WP_001270417.1
Coordinates 6837..7124 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag RG61_RS26615 Protein ID WP_001132895.1
Coordinates 6589..6840 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG61_RS29175 1958..2062 - 105 Protein_2 aerotaxis sensor receptor, flavoprotein -
RG61_RS26575 2494..2562 + 69 Protein_3 DUF4113 domain-containing protein -
RG61_RS28505 2684..3440 - 757 Protein_4 IS4 family transposase -
RG61_RS26590 3674..4636 - 963 WP_000756325.1 ParB/RepB/Spo0J family partition protein -
RG61_RS26595 4633..5838 - 1206 WP_000817644.1 AAA family ATPase -
RG61_RS26600 6037..6222 - 186 WP_073519882.1 hypothetical protein -
RG61_RS26605 6212..6424 + 213 WP_032334873.1 hypothetical protein -
RG61_RS26610 6393..6599 - 207 WP_073519883.1 hypothetical protein -
RG61_RS26615 6589..6840 + 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RG61_RS26620 6837..7124 + 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG61_RS29180 7123..7403 + 281 Protein_12 transposase -
RG61_RS26640 8053..9030 + 978 WP_000361618.1 RepB family plasmid replication initiator protein -
RG61_RS26645 9315..10055 - 741 WP_073519885.1 site-specific integrase -
RG61_RS26650 10218..10364 - 147 WP_073519886.1 serine/threonine protein kinase -
RG61_RS26655 10726..10973 + 248 Protein_16 hypothetical protein -
RG61_RS26660 11046..11894 + 849 WP_073519985.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - faeI / faeH / faeF / faeE / faeD / faeC / pic 1..146496 146496


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T51413 WP_001270417.1 NZ_CP010214:6837-7124 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T51413 NZ_CP010214:6837-7124 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGATAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGAAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT51413 WP_001132895.1 NZ_CP010214:6589-6840 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT51413 NZ_CP010214:6589-6840 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCCATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References