Detailed information of TA system TA051412

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5072692..5073527
• Replicon: chromosome
• Accession: NZ_CP010213
• Organism: Escherichia coli strain M15

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E3PD61
• Locus tag: RG61_RS26385
• Protein ID: WP_001094449.1
• Coordinates: 5072692..5073069 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG61_RS26390
• Protein ID: WP_073519839.1
• Coordinates: 5073159..5073527 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG61_RS26360	5068332..5069255	-	924	Protein_4799	DMT family transporter	-
RG61_RS26365	5069366..5070550	-	1185	WP_001172876.1	sugar efflux transporter	-
RG61_RS26370	5070932..5071078	-	147	WP_001315909.1	hypothetical protein	-
RG61_RS26375	5071343..5072188	-	846	WP_061892569.1	DUF4942 domain-containing protein	-
RG61_RS29160	5072273..5072470	-	198	WP_087891818.1	DUF957 domain-containing protein	-
RG61_RS29165	5072546..5072695	-	150	Protein_4804	hypothetical protein	-
RG61_RS26385	5072692..5073069	-	378	WP_001094449.1	TA system toxin CbtA family protein	Toxin
RG61_RS26390	5073159..5073527	-	369	WP_073519839.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG61_RS26395	5073607..5073828	-	222	WP_001546495.1	DUF987 domain-containing protein	-
RG61_RS26400	5073885..5074361	-	477	WP_061892568.1	RadC family protein	-
RG61_RS26405	5074377..5074856	-	480	WP_061892567.1	antirestriction protein	-
RG61_RS28485	5074955..5075155	+	201	WP_077768309.1	hypothetical protein	-
RG61_RS26415	5075173..5075991	-	819	WP_061892566.1	DUF945 domain-containing protein	-
RG61_RS29170	5076082..5076239	-	158	Protein_4812	septum formation initiator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14162.00 Da
• Isoelectric Point: 6.6312

>T51412 WP_001094449.1 NZ_CP010213:c5073069-5072692 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SSCTHSQLINSIDILRARRATGLMTRDNYRTVNDITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T51412 NZ_CP010213:c5073069-5072692 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCAGCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13837.70 Da
• Isoelectric Point: 6.2066

>AT51412 WP_073519839.1 NZ_CP010213:c5073527-5073159 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLICEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT51412 NZ_CP010213:c5073527-5073159 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGATCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: E3PD61