Detailed information of TA system TA051410

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4549320..4549817
• Replicon: chromosome
• Accession: NZ_CP010213
• Organism: Escherichia coli strain M15

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: RG61_RS23675
• Protein ID: WP_001173343.1
• Coordinates: 4549320..4549493 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: RG61_RS23680
• Protein ID: WP_073519819.1
• Coordinates: 4549521..4549817 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG61_RS23665	4545990..4547447	+	1458	WP_000856832.1	dipeptide/tripeptide permease DtpC	-
RG61_RS23670	4547684..4549201	+	1518	WP_001295074.1	lysine--tRNA ligase	-
RG61_RS23675	4549320..4549493	-	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
RG61_RS23680	4549521..4549817	-	297	WP_073519819.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
RG61_RS23690	4550044..4550316	-	273	WP_000405647.1	N-acetyltransferase	-
RG61_RS23695	4550328..4550558	-	231	WP_000371700.1	(4Fe-4S)-binding protein	-
RG61_RS23700	4550739..4552370	+	1632	WP_001216487.1	sensor histidine kinase	-
RG61_RS23705	4552367..4553086	+	720	WP_000611288.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T51410 WP_001173343.1 NZ_CP010213:c4549493-4549320 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T51410 NZ_CP010213:c4549493-4549320 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11468.96 Da
• Isoelectric Point: 4.0994

>AT51410 WP_073519819.1 NZ_CP010213:c4549817-4549521 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNEDSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT51410 NZ_CP010213:c4549817-4549521 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAGATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41