Detailed information of TA system TA051392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1443971..1444596
• Replicon: chromosome
• Accession: NZ_CP010213
• Organism: Escherichia coli strain M15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RG61_RS07535
• Protein ID: WP_000911330.1
• Coordinates: 1444198..1444596 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: RG61_RS07530
• Protein ID: WP_000450524.1
• Coordinates: 1443971..1444198 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG61_RS07500	1439774..1440244	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
RG61_RS07505	1440244..1440816	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
RG61_RS07510	1440962..1441840	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
RG61_RS07515	1441857..1442891	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
RG61_RS07525	1443104..1443817	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
RG61_RS07530	1443971..1444198	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
RG61_RS07535	1444198..1444596	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
RG61_RS07540	1444743..1445606	+	864	Protein_1387	neutral zinc metallopeptidase	-
RG61_RS07545	1445621..1447636	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
RG61_RS07550	1447710..1448408	+	699	WP_000679823.1	esterase	-
RG61_RS07555	1448518..1448718	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T51392 WP_000911330.1 NZ_CP010213:1444198-1444596 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T51392 NZ_CP010213:1444198-1444596 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT51392 WP_000450524.1 NZ_CP010213:1443971-1444198 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT51392 NZ_CP010213:1443971-1444198 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33