Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 997481..998135 Replicon chromosome
Accession NZ_CP010213
Organism Escherichia coli strain M15

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag RG61_RS05265 Protein ID WP_000244772.1
Coordinates 997728..998135 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG61_RS05260 Protein ID WP_000354046.1
Coordinates 997481..997747 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG61_RS05235 992650..993393 + 744 WP_000951964.1 SDR family oxidoreductase -
RG61_RS05240 993450..994883 - 1434 WP_141221849.1 6-phospho-beta-glucosidase BglA -
RG61_RS05245 994928..995239 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
RG61_RS05250 995403..996062 + 660 WP_000250274.1 hemolysin III family protein -
RG61_RS05255 996258..997238 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
RG61_RS05260 997481..997747 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG61_RS05265 997728..998135 + 408 WP_000244772.1 protein YgfX Toxin
RG61_RS05270 998175..998696 - 522 WP_001055874.1 flavodoxin FldB -
RG61_RS05275 998808..999704 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG61_RS05280 999729..1000439 + 711 WP_073519527.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG61_RS05285 1000445..1002178 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T51390 WP_000244772.1 NZ_CP010213:997728-998135 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T51390 NZ_CP010213:997728-998135 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT51390 WP_000354046.1 NZ_CP010213:997481-997747 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT51390 NZ_CP010213:997481-997747 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References