Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 73825..74360 Replicon plasmid A
Accession NZ_CP010207
Organism Escherichia coli strain M11

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag RG60_RS26705 Protein ID WP_001270417.1
Coordinates 73825..74112 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag RG60_RS26710 Protein ID WP_001132895.1
Coordinates 74109..74360 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG60_RS26665 69055..69903 - 849 WP_073519985.1 hypothetical protein -
RG60_RS26670 69976..70223 - 248 Protein_88 hypothetical protein -
RG60_RS26675 70585..70731 + 147 WP_073519886.1 serine/threonine protein kinase -
RG60_RS26680 70894..71634 + 741 WP_073519885.1 site-specific integrase -
RG60_RS26685 71919..72896 - 978 WP_000361618.1 RepB family plasmid replication initiator protein -
RG60_RS29175 73546..73826 - 281 Protein_92 transposase -
RG60_RS26705 73825..74112 - 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG60_RS26710 74109..74360 - 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RG60_RS29180 74350..74556 + 207 WP_073519883.1 hypothetical protein -
RG60_RS26715 74525..74737 - 213 WP_032334873.1 hypothetical protein -
RG60_RS26720 74727..74912 + 186 WP_073519882.1 hypothetical protein -
RG60_RS26725 75111..76316 + 1206 WP_000817644.1 AAA family ATPase -
RG60_RS26730 76313..77275 + 963 WP_000756325.1 ParB/RepB/Spo0J family partition protein -
RG60_RS28475 77509..78265 + 757 Protein_100 IS4 family transposase -
RG60_RS26745 78387..78455 - 69 Protein_101 DUF4113 domain-containing protein -
RG60_RS29185 78887..78991 + 105 Protein_102 aerotaxis sensor receptor, flavoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pic / faeC / faeD / faeE / faeF / faeH / faeI 1..138950 138950


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T51380 WP_001270417.1 NZ_CP010207:c74112-73825 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T51380 NZ_CP010207:c74112-73825 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGATAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGAAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT51380 WP_001132895.1 NZ_CP010207:c74360-74109 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT51380 NZ_CP010207:c74360-74109 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCCATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References