Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3723764..3724382 Replicon chromosome
Accession NZ_CP010200
Organism Escherichia coli strain M10

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag RG59_RS19290 Protein ID WP_001291435.1
Coordinates 3723764..3723982 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag RG59_RS19295 Protein ID WP_000344800.1
Coordinates 3724008..3724382 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG59_RS19255 3719054..3719626 + 573 WP_000779831.1 YbaY family lipoprotein -
RG59_RS19260 3719657..3720046 - 390 WP_001300427.1 DNA base-flipping protein -
RG59_RS19270 3720347..3720700 + 354 WP_000878140.1 DUF1428 domain-containing protein -
RG59_RS19275 3720742..3722292 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
RG59_RS19280 3722456..3722926 - 471 WP_000136192.1 YlaC family protein -
RG59_RS19285 3723042..3723593 - 552 WP_000102564.1 maltose O-acetyltransferase -
RG59_RS19290 3723764..3723982 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
RG59_RS19295 3724008..3724382 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
RG59_RS19300 3724928..3728077 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
RG59_RS19305 3728100..3729293 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T51346 WP_001291435.1 NZ_CP010200:c3723982-3723764 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T51346 NZ_CP010200:c3723982-3723764 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT51346 WP_000344800.1 NZ_CP010200:c3724382-3724008 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT51346 NZ_CP010200:c3724382-3724008 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References