Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2166480..2167111 Replicon chromosome
Accession NZ_CP010200
Organism Escherichia coli strain M10

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag RG59_RS11390 Protein ID WP_001259385.1
Coordinates 2166836..2167111 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag RG59_RS11385 Protein ID WP_000593554.1
Coordinates 2166480..2166839 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG59_RS11355 2161592..2162065 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
RG59_RS11360 2162062..2162343 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
RG59_RS11365 2162420..2163778 + 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
RG59_RS11370 2163771..2165279 + 1509 WP_001298718.1 carbohydrate kinase -
RG59_RS11375 2165269..2165538 + 270 WP_000933060.1 HPr family phosphocarrier protein -
RG59_RS11380 2165570..2166430 + 861 WP_001131783.1 class II aldolase -
RG59_RS11385 2166480..2166839 - 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
RG59_RS11390 2166836..2167111 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
RG59_RS11395 2167184..2168308 - 1125 WP_001314210.1 ABC transporter permease -
RG59_RS11400 2168308..2171043 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
RG59_RS11405 2171040..2172107 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T51338 WP_001259385.1 NZ_CP010200:c2167111-2166836 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T51338 NZ_CP010200:c2167111-2166836 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT51338 WP_000593554.1 NZ_CP010200:c2166839-2166480 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT51338 NZ_CP010200:c2166839-2166480 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References