Detailed information of TA system TA051333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1095708..1096333
• Replicon: chromosome
• Accession: NZ_CP010200
• Organism: Escherichia coli strain M10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RG59_RS05715
• Protein ID: WP_000911330.1
• Coordinates: 1095708..1096106 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: RG59_RS05720
• Protein ID: WP_000450524.1
• Coordinates: 1096106..1096333 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG59_RS05695	1091586..1091786	+	201	WP_000383836.1	YpfN family protein	-
RG59_RS05700	1091896..1092594	-	699	WP_000679823.1	esterase	-
RG59_RS05705	1092668..1094683	-	2016	WP_000829355.1	tRNA cytosine(34) acetyltransferase TmcA	-
RG59_RS05710	1094698..1095561	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
RG59_RS05715	1095708..1096106	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
RG59_RS05720	1096106..1096333	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
RG59_RS05725	1096487..1097200	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
RG59_RS05735	1097413..1098447	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
RG59_RS05740	1098464..1099342	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
RG59_RS05745	1099488..1100060	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
RG59_RS05750	1100060..1100530	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T51333 WP_000911330.1 NZ_CP010200:c1096106-1095708 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T51333 NZ_CP010200:c1096106-1095708 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT51333 WP_000450524.1 NZ_CP010200:c1096333-1096106 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT51333 NZ_CP010200:c1096333-1096106 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33