Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 127565..128100 Replicon plasmid A
Accession NZ_CP010197
Organism Escherichia coli strain M9

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag RG58_RS27645 Protein ID WP_001270417.1
Coordinates 127565..127852 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag RG58_RS27650 Protein ID WP_001132895.1
Coordinates 127849..128100 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG58_RS27605 122794..123643 - 850 Protein_137 hypothetical protein -
RG58_RS27615 124325..124471 + 147 WP_073519886.1 serine/threonine protein kinase -
RG58_RS27620 124634..125374 + 741 WP_073519885.1 site-specific integrase -
RG58_RS27625 125659..126636 - 978 WP_000361618.1 RepB family plasmid replication initiator protein -
RG58_RS29020 127286..127566 - 281 Protein_141 integrase core domain-containing protein -
RG58_RS27645 127565..127852 - 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG58_RS27650 127849..128100 - 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RG58_RS27655 128265..128477 - 213 WP_032334873.1 hypothetical protein -
RG58_RS27665 128851..130056 + 1206 WP_000817644.1 AAA family ATPase -
RG58_RS27670 130053..131015 + 963 WP_000756325.1 ParB/RepB/Spo0J family partition protein -
RG58_RS28370 131249..132005 + 757 Protein_147 IS4 family transposase -
RG58_RS29320 132119..132594 + 476 Protein_148 DUF1281 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pic / faeC / faeD / faeE / faeF / faeH / faeI 1..146524 146524


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T51323 WP_001270417.1 NZ_CP010197:c127852-127565 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T51323 NZ_CP010197:c127852-127565 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGATAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGAAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT51323 WP_001132895.1 NZ_CP010197:c128100-127849 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT51323 NZ_CP010197:c128100-127849 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCCATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References