Detailed information of TA system TA051317

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5111143..5111978
• Replicon: chromosome
• Accession: NZ_CP010196
• Organism: Escherichia coli strain M9

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E3PD61
• Locus tag: RG58_RS26645
• Protein ID: WP_001094449.1
• Coordinates: 5111143..5111520 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG58_RS26650
• Protein ID: WP_073519839.1
• Coordinates: 5111610..5111978 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG58_RS26615	5106783..5107706	-	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-
RG58_RS26620	5107817..5109001	-	1185	WP_001172876.1	sugar efflux transporter	-
RG58_RS26635	5109794..5110639	-	846	WP_061892569.1	DUF4942 domain-containing protein	-
RG58_RS28930	5110724..5110921	-	198	WP_087891818.1	DUF957 domain-containing protein	-
RG58_RS28935	5110997..5111146	-	150	Protein_4788	hypothetical protein	-
RG58_RS26645	5111143..5111520	-	378	WP_001094449.1	TA system toxin CbtA family protein	Toxin
RG58_RS26650	5111610..5111978	-	369	WP_073519839.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG58_RS26655	5112058..5112279	-	222	WP_001546495.1	DUF987 domain-containing protein	-
RG58_RS26660	5112336..5112812	-	477	WP_061892568.1	RadC family protein	-
RG58_RS26665	5112828..5113307	-	480	WP_061892567.1	antirestriction protein	-
RG58_RS26675	5113624..5114442	-	819	WP_061892566.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14162.00 Da
• Isoelectric Point: 6.6312

>T51317 WP_001094449.1 NZ_CP010196:c5111520-5111143 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SSCTHSQLINSIDILRARRATGLMTRDNYRTVNDITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T51317 NZ_CP010196:c5111520-5111143 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCAGCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13837.70 Da
• Isoelectric Point: 6.2066

>AT51317 WP_073519839.1 NZ_CP010196:c5111978-5111610 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLICEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT51317 NZ_CP010196:c5111978-5111610 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGATCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: E3PD61