Detailed information of TA system TA051315

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4587773..4588270
• Replicon: chromosome
• Accession: NZ_CP010196
• Organism: Escherichia coli strain M9

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: RG58_RS23935
• Protein ID: WP_001173343.1
• Coordinates: 4587773..4587946 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: RG58_RS23940
• Protein ID: WP_073519819.1
• Coordinates: 4587974..4588270 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG58_RS23925	4584443..4585900	+	1458	WP_000856832.1	dipeptide/tripeptide permease DtpC	-
RG58_RS23930	4586137..4587654	+	1518	WP_001295074.1	lysine--tRNA ligase	-
RG58_RS23935	4587773..4587946	-	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
RG58_RS23940	4587974..4588270	-	297	WP_073519819.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
RG58_RS23950	4588497..4588769	-	273	WP_000405647.1	N-acetyltransferase	-
RG58_RS23955	4588781..4589011	-	231	WP_000371700.1	(4Fe-4S)-binding protein	-
RG58_RS23960	4589192..4590823	+	1632	WP_001216487.1	sensor histidine kinase	-
RG58_RS23965	4590820..4591539	+	720	WP_000611288.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T51315 WP_001173343.1 NZ_CP010196:c4587946-4587773 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T51315 NZ_CP010196:c4587946-4587773 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11468.96 Da
• Isoelectric Point: 4.0994

>AT51315 WP_073519819.1 NZ_CP010196:c4588270-4587974 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNEDSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT51315 NZ_CP010196:c4588270-4587974 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAGATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41