Detailed information of TA system TA051308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2550613..2551238
• Replicon: chromosome
• Accession: NZ_CP010196
• Organism: Escherichia coli strain M9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RG58_RS13355
• Protein ID: WP_000911330.1
• Coordinates: 2550613..2551011 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: RG58_RS13360
• Protein ID: WP_000450524.1
• Coordinates: 2551011..2551238 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG58_RS13335	2546491..2546691	+	201	WP_000383836.1	YpfN family protein	-
RG58_RS13340	2546801..2547499	-	699	WP_000679823.1	esterase	-
RG58_RS13345	2547573..2549588	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
RG58_RS13350	2549603..2550466	-	864	Protein_2427	neutral zinc metallopeptidase	-
RG58_RS13355	2550613..2551011	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
RG58_RS13360	2551011..2551238	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
RG58_RS13365	2551392..2552105	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
RG58_RS13375	2552318..2553352	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
RG58_RS13380	2553369..2554247	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
RG58_RS13385	2554393..2554965	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
RG58_RS13390	2554965..2555435	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T51308 WP_000911330.1 NZ_CP010196:c2551011-2550613 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T51308 NZ_CP010196:c2551011-2550613 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT51308 WP_000450524.1 NZ_CP010196:c2551238-2551011 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT51308 NZ_CP010196:c2551238-2551011 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33