Detailed information of TA system TA051297

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 122133..122968
• Replicon: chromosome
• Accession: NZ_CP010196
• Organism: Escherichia coli strain M9

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG58_RS00615
• Protein ID: WP_049590183.1
• Coordinates: 122591..122968 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG58_RS00610
• Protein ID: WP_049590182.1
• Coordinates: 122133..122501 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG58_RS00565	118259..118714	+	456	WP_000581502.1	hypothetical protein	-
RG58_RS00580	118998..119807	+	810	Protein_99	DUF945 domain-containing protein	-
RG58_RS00590	120156..120635	+	480	WP_000706981.1	antirestriction protein	-
RG58_RS00595	120651..121127	+	477	WP_001186724.1	RadC family protein	-
RG58_RS00600	121196..121417	+	222	WP_000692300.1	DUF987 domain-containing protein	-
RG58_RS00605	121436..122083	+	648	WP_049590180.1	hypothetical protein	-
RG58_RS00610	122133..122501	+	369	WP_049590182.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG58_RS00615	122591..122968	+	378	WP_049590183.1	TA system toxin CbtA family protein	Toxin
RG58_RS00620	122965..123453	+	489	WP_000761700.1	hypothetical protein	-
RG58_RS00625	123473..123670	+	198	WP_000772024.1	DUF957 domain-containing protein	-
RG58_RS00630	123767..124615	+	849	WP_049590184.1	DUF4942 domain-containing protein	-
RG58_RS00635	124708..126027	-	1320	WP_049590185.1	site-specific integrase	-
RG58_RS00640	126365..126703	-	339	Protein_110	LysR family transcriptional regulator	-
RG58_RS00645	126754..127710	-	957	WP_000121330.1	molybdenum cofactor insertion chaperone PaoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	121436..132589	11153

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13984.01 Da
• Isoelectric Point: 9.6545

>T51297 WP_049590183.1 NZ_CP010196:122591-122968 [Escherichia coli]
MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITRGKHPEAKR

Nucleotide

• Length: 378 bp

>T51297 NZ_CP010196:122591-122968 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGTCGGCCAGTTCACGCCCATCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCGGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13710.64 Da
• Isoelectric Point: 6.9371

>AT51297 WP_049590182.1 NZ_CP010196:122133-122501 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRASIRGRFRDADAYHLDQAFPLLMKQLKLMLTSG
ELNPCHQHTVTLYARGLICEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT51297 NZ_CP010196:122133-122501 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCAGTATCAGAGGCCGGT
TCAGGGACGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACTAGCGGT
GAACTGAATCCCTGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGATCTGCGAAGCCGACACCTTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA