Detailed information of TA system TA051290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 3682..4217
• Replicon: plasmid A
• Accession: NZ_CP010192
• Organism: Escherichia coli strain M8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: RG57_RS25040
• Protein ID: WP_001270421.1
• Coordinates: 3930..4217 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: RG57_RS25035
• Protein ID: WP_001132895.1
• Coordinates: 3682..3933 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG57_RS25000	111..569	-	459	Protein_0	IS200/IS605 family transposase	-
RG57_RS25010	758..1720	-	963	WP_077898678.1	ParB/RepB/Spo0J family partition protein	-
RG57_RS25015	1717..2922	-	1206	WP_000817642.1	AAA family ATPase	-
RG57_RS25025	3293..3508	+	216	WP_077898679.1	hypothetical protein	-
RG57_RS25035	3682..3933	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RG57_RS25040	3930..4217	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG57_RS25045	4219..4630	+	412	Protein_6	DDE-type integrase/transposase/recombinase	-
RG57_RS25060	5275..6252	+	978	WP_001355280.1	RepB family plasmid replication initiator protein	-
RG57_RS25065	6537..7277	-	741	WP_001066926.1	tyrosine-type recombinase/integrase	-
RG57_RS25070	7398..7559	-	162	WP_073535365.1	transcriptional regulator	-
RG57_RS26945	7871..8823	-	953	Protein_10	IS630 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..162720	162720
	-	flank	IS/Tn	-	-	111..356	245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T51290 WP_001270421.1 NZ_CP010192:3930-4217 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T51290 NZ_CP010192:3930-4217 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT51290 WP_001132895.1 NZ_CP010192:3682-3933 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT51290 NZ_CP010192:3682-3933 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6