Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2857608..2858233 Replicon chromosome
Accession NZ_CP010191
Organism Escherichia coli strain M8

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag RG57_RS14715 Protein ID WP_000911329.1
Coordinates 2857835..2858233 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag RG57_RS14710 Protein ID WP_000450524.1
Coordinates 2857608..2857835 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG57_RS14680 2853410..2853880 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
RG57_RS14685 2853880..2854452 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
RG57_RS14690 2854598..2855476 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
RG57_RS14695 2855493..2856527 + 1035 WP_001440783.1 outer membrane protein assembly factor BamC -
RG57_RS14705 2856740..2857453 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
RG57_RS14710 2857608..2857835 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
RG57_RS14715 2857835..2858233 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
RG57_RS14720 2858380..2859243 + 864 WP_001267498.1 neutral zinc metallopeptidase -
RG57_RS14725 2859258..2861273 + 2016 WP_047084717.1 tRNA cytosine(34) acetyltransferase TmcA -
RG57_RS14730 2861347..2862045 + 699 WP_000679812.1 esterase -
RG57_RS14735 2862126..2862326 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T51275 WP_000911329.1 NZ_CP010191:2857835-2858233 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T51275 NZ_CP010191:2857835-2858233 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT51275 WP_000450524.1 NZ_CP010191:2857608-2857835 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT51275 NZ_CP010191:2857608-2857835 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References