Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2557427..2558010 Replicon chromosome
Accession NZ_CP010191
Organism Escherichia coli strain M8

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag RG57_RS13185 Protein ID WP_000254745.1
Coordinates 2557675..2558010 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2J1DJJ7
Locus tag RG57_RS13180 Protein ID WP_000581939.1
Coordinates 2557427..2557675 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG57_RS13170 2553766..2555067 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
RG57_RS13175 2555115..2557349 + 2235 WP_000226815.1 GTP pyrophosphokinase -
RG57_RS13180 2557427..2557675 + 249 WP_000581939.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
RG57_RS13185 2557675..2558010 + 336 WP_000254745.1 endoribonuclease MazF Toxin
RG57_RS13190 2558081..2558872 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
RG57_RS13195 2559100..2560737 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
RG57_RS13200 2560825..2562123 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

Antitoxin PemI-like

(2-82)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T51274 WP_000254745.1 NZ_CP010191:2557675-2558010 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T51274 NZ_CP010191:2557675-2558010 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9369.76 Da        Isoelectric Point: 4.4791

>AT51274 WP_000581939.1 NZ_CP010191:2557427-2557675 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNEITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT51274 NZ_CP010191:2557427-2557675 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGAGATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A2J1DJJ7

References