Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 127564..128099 Replicon plasmid A
Accession NZ_CP010187
Organism Escherichia coli strain M6

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag RG56_RS27650 Protein ID WP_001270417.1
Coordinates 127564..127851 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag RG56_RS27655 Protein ID WP_001132895.1
Coordinates 127848..128099 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG56_RS27610 122793..123642 - 850 Protein_137 hypothetical protein -
RG56_RS27620 124324..124470 + 147 WP_073519886.1 serine/threonine protein kinase -
RG56_RS27625 124633..125373 + 741 WP_073519885.1 site-specific integrase -
RG56_RS27630 125658..126635 - 978 WP_000361618.1 RepB family plasmid replication initiator protein -
RG56_RS29085 127285..127565 - 281 Protein_141 integrase core domain-containing protein -
RG56_RS27650 127564..127851 - 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG56_RS27655 127848..128099 - 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RG56_RS27660 128264..128476 - 213 WP_032334873.1 hypothetical protein -
RG56_RS27670 128850..130055 + 1206 WP_000817644.1 AAA family ATPase -
RG56_RS27675 130052..131014 + 963 WP_000756325.1 ParB/RepB/Spo0J family partition protein -
RG56_RS28410 131248..132004 + 757 Protein_147 IS4 family transposase -
RG56_RS29390 132118..132593 + 476 Protein_148 DUF1281 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pic / faeC / faeD / faeE / faeF / faeH / faeI 1..146575 146575


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T51266 WP_001270417.1 NZ_CP010187:c127851-127564 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T51266 NZ_CP010187:c127851-127564 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGATAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGAAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT51266 WP_001132895.1 NZ_CP010187:c128099-127848 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT51266 NZ_CP010187:c128099-127848 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCCATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References