Detailed information of TA system TA051265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 75224..75705
• Replicon: plasmid A
• Accession: NZ_CP010187
• Organism: Escherichia coli strain M6

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: RG56_RS27350
• Protein ID: WP_073519917.1
• Coordinates: 75490..75705 (+)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: RG56_RS27345
• Protein ID: WP_073519918.1
• Coordinates: 75224..75493 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG56_RS29375	70491..70685	-	195	WP_157916191.1	hypothetical protein	-
RG56_RS27285	70925..71385	+	461	Protein_83	thermonuclease family protein	-
RG56_RS27290	71431..71640	+	210	WP_032334801.1	hemolysin expression modulator Hha	-
RG56_RS27295	71678..72268	+	591	WP_032334802.1	DUF2726 domain-containing protein	-
-	72441..72497	-	57	NuclAT_1	-	-
-	72441..72497	-	57	NuclAT_1	-	-
-	72441..72497	-	57	NuclAT_1	-	-
-	72441..72497	-	57	NuclAT_1	-	-
RG56_RS27305	72546..72695	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	-
RG56_RS27310	72896..73153	+	258	WP_073519922.1	replication regulatory protein RepA	-
RG56_RS27320	73385..73459	+	75	WP_001365705.1	RepA leader peptide Tap	-
RG56_RS27325	73452..74309	+	858	WP_073528052.1	incFII family plasmid replication initiator RepA	-
RG56_RS29380	75011..75169	+	159	WP_073519919.1	hypothetical protein	-
RG56_RS27345	75224..75493	+	270	WP_073519918.1	hypothetical protein	Antitoxin
RG56_RS27350	75490..75705	+	216	WP_073519917.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG56_RS27355	75857..76162	+	306	WP_073521420.1	hypothetical protein	-
RG56_RS27360	76159..76431	-	273	WP_046201741.1	hypothetical protein	-
RG56_RS29385	76424..76747	-	324	Protein_95	recombinase family protein	-
RG56_RS27380	78030..78665	+	636	WP_073519914.1	CPBP family intramembrane metalloprotease	-
RG56_RS27385	78768..80021	+	1254	WP_073519913.1	HlyD family efflux transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic / faeC / faeD / faeE / faeF / faeH / faeI	1..146575	146575

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-64)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 8017.24 Da
• Isoelectric Point: 6.4468

>T51265 WP_073519917.1 NZ_CP010187:75490-75705 [Escherichia coli]
MMEIFCTMLASQDRKRIREYIAEQNLMAAIELDELIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFCSGL

Nucleotide

• Length: 216 bp

>T51265 NZ_CP010187:75490-75705 [Escherichia coli]
ATGATGGAGATATTCTGTACTATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACTGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCCCATTTTTGTTCTGGTTTATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.62 Da
• Isoelectric Point: 6.4710

>AT51265 WP_073519918.1 NZ_CP010187:75224-75493 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEEGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT51265 NZ_CP010187:75224-75493 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGAAGGACGTCAGCAACTCGAGCGCGGTGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA