Detailed information of TA system TA051260

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5081577..5082375
• Replicon: chromosome
• Accession: NZ_CP010186
• Organism: Escherichia coli strain M6

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG56_RS26460
• Protein ID: WP_021573868.1
• Coordinates: 5081577..5081954 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG56_RS26465
• Protein ID: WP_073519787.1
• Coordinates: 5082001..5082375 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG56_RS26425	5077611..5077902	+	292	Protein_4755	transposase	-
RG56_RS26430	5077899..5078249	+	351	WP_000624707.1	IS66 family insertion sequence element accessory protein TnpB	-
RG56_RS26435	5078280..5079849	+	1570	Protein_4757	IS66 family transposase	-
RG56_RS26445	5080021..5081009	+	989	Protein_4758	IS630 family transposase	-
RG56_RS26455	5081088..5081580	-	493	Protein_4759	hypothetical protein	-
RG56_RS26460	5081577..5081954	-	378	WP_021573868.1	TA system toxin CbtA family protein	Toxin
RG56_RS26465	5082001..5082375	-	375	WP_073519787.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG56_RS26470	5082425..5083069	-	645	WP_073519465.1	antitoxin of toxin-antitoxin stability system	-
RG56_RS26475	5083088..5083309	-	222	WP_000692315.1	DUF987 domain-containing protein	-
RG56_RS26480	5083372..5083848	-	477	WP_001186193.1	RadC family protein	-
RG56_RS26485	5083864..5084337	-	474	WP_001313575.1	antirestriction protein	-
RG56_RS26490	5084431..5084676	-	246	WP_001164966.1	hypothetical protein	-
RG56_RS26495	5084676..5085497	-	822	WP_021567056.1	DUF945 domain-containing protein	-
RG56_RS26505	5085597..5085830	-	234	WP_001213776.1	DUF905 family protein	-
RG56_RS26510	5085948..5086400	-	453	WP_000682723.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14299.33 Da
• Isoelectric Point: 6.8531

>T51260 WP_021573868.1 NZ_CP010186:c5081954-5081577 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILLTRRATGLMTRSNYRMVNDIIRGEHSEAKR

Nucleotide

• Length: 378 bp

>T51260 NZ_CP010186:c5081954-5081577 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCACGTTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACTCTGAATGACACACCATTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATTCTCCTGACCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13880.67 Da
• Isoelectric Point: 6.3118

>AT51260 WP_073519787.1 NZ_CP010186:c5082375-5082001 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRASIRGRFRDADAYHLDQAFPLLMKQLKLMLTSG
ELNPCHQHTVTLYAKGLTCEADTFGSCGYVYLAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT51260 NZ_CP010186:c5082375-5082001 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCAGTATCAGAGGCCGGT
TCAGGGACGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGCGGT
GAACTGAATCCCTGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCTTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACTGGCACCCGCAACCACTTCATAA