Detailed information of TA system TA051258

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4551588..4552085
• Replicon: chromosome
• Accession: NZ_CP010186
• Organism: Escherichia coli strain M6

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: RG56_RS23785
• Protein ID: WP_001173343.1
• Coordinates: 4551912..4552085 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: RG56_RS23780
• Protein ID: WP_073519819.1
• Coordinates: 4551588..4551884 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG56_RS23755	4548319..4549038	-	720	WP_000611288.1	two-component system response regulator DcuR	-
RG56_RS23760	4549035..4550666	-	1632	WP_001216487.1	sensor histidine kinase	-
RG56_RS23765	4550847..4551077	+	231	WP_000371700.1	(4Fe-4S)-binding protein	-
RG56_RS23770	4551089..4551361	+	273	WP_000405647.1	N-acetyltransferase	-
RG56_RS23780	4551588..4551884	+	297	WP_073519819.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
RG56_RS23785	4551912..4552085	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
RG56_RS23790	4552204..4553721	-	1518	WP_001295074.1	lysine--tRNA ligase	-
RG56_RS23795	4553958..4555415	-	1458	WP_000856832.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T51258 WP_001173343.1 NZ_CP010186:4551912-4552085 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T51258 NZ_CP010186:4551912-4552085 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11468.96 Da
• Isoelectric Point: 4.0994

>AT51258 WP_073519819.1 NZ_CP010186:4551588-4551884 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNEDSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT51258 NZ_CP010186:4551588-4551884 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAGATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41