Detailed information of TA system TA051256

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4027883..4028718
• Replicon: chromosome
• Accession: NZ_CP010186
• Organism: Escherichia coli strain M6

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E3PD61
• Locus tag: RG56_RS21085
• Protein ID: WP_001094449.1
• Coordinates: 4028341..4028718 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: RG56_RS21080
• Protein ID: WP_073519839.1
• Coordinates: 4027883..4028251 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG56_RS21055	4025419..4026237	+	819	WP_061892566.1	DUF945 domain-containing protein	-
RG56_RS21065	4026554..4027033	+	480	WP_061892567.1	antirestriction protein	-
RG56_RS21070	4027049..4027525	+	477	WP_061892568.1	RadC family protein	-
RG56_RS21075	4027582..4027803	+	222	WP_001546495.1	DUF987 domain-containing protein	-
RG56_RS21080	4027883..4028251	+	369	WP_073519839.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG56_RS21085	4028341..4028718	+	378	WP_001094449.1	TA system toxin CbtA family protein	Toxin
RG56_RS28920	4028715..4028864	+	150	Protein_3816	hypothetical protein	-
RG56_RS28925	4028940..4029137	+	198	WP_087891818.1	DUF957 domain-containing protein	-
RG56_RS21095	4029222..4030067	+	846	WP_061892569.1	DUF4942 domain-containing protein	-
RG56_RS21105	4030860..4032044	+	1185	WP_001172876.1	sugar efflux transporter	-
RG56_RS21110	4032155..4033078	+	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14162.00 Da
• Isoelectric Point: 6.6312

>T51256 WP_001094449.1 NZ_CP010186:4028341-4028718 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SSCTHSQLINSIDILRARRATGLMTRDNYRTVNDITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T51256 NZ_CP010186:4028341-4028718 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCAGCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13837.70 Da
• Isoelectric Point: 6.2066

>AT51256 WP_073519839.1 NZ_CP010186:4027883-4028251 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLICEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT51256 NZ_CP010186:4027883-4028251 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGATCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: E3PD61