Detailed information of TA system TA051239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 28171..28706
• Replicon: plasmid A
• Accession: NZ_CP010184
• Organism: Escherichia coli strain M3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: RG55_RS26090
• Protein ID: WP_001270421.1
• Coordinates: 28419..28706 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: RG55_RS26085
• Protein ID: WP_001132895.1
• Coordinates: 28171..28422 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG55_RS26055	23725..24894	+	1170	WP_046201818.1	transposase	-
RG55_RS26060	24934..25107	+	174	Protein_26	DUF4113 domain-containing protein	-
RG55_RS26065	25248..26210	-	963	WP_046201819.1	ParB/RepB/Spo0J family partition protein	-
RG55_RS26070	26207..27412	-	1206	WP_000817642.1	AAA family ATPase	-
RG55_RS26080	27786..27998	+	213	WP_000402944.1	hypothetical protein	-
RG55_RS26085	28171..28422	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RG55_RS26090	28419..28706	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG55_RS26095	28720..28979	+	260	Protein_32	hypothetical protein	-
RG55_RS26100	28992..29282	+	291	WP_046201820.1	hypothetical protein	-
RG55_RS28215	29329..29696	+	368	Protein_34	DDE-type integrase/transposase/recombinase	-
RG55_RS26120	30340..31317	+	978	WP_000361612.1	RepB family plasmid replication initiator protein	-
RG55_RS26125	31596..32336	-	741	WP_046201823.1	tyrosine-type recombinase/integrase	-
RG55_RS26130	32457..32618	-	162	WP_064756529.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic	1..200925	200925
	-	inside	IScluster/Tn	-	-	19048..33882	14834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T51239 WP_001270421.1 NZ_CP010184:28419-28706 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T51239 NZ_CP010184:28419-28706 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTGATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT51239 WP_001132895.1 NZ_CP010184:28171-28422 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT51239 NZ_CP010184:28171-28422 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAACTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6