Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4640013..4640651 Replicon chromosome
Accession NZ_CP010183
Organism Escherichia coli strain M3

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag RG55_RS24335 Protein ID WP_000813794.1
Coordinates 4640475..4640651 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RG55_RS24330 Protein ID WP_001270286.1
Coordinates 4640013..4640429 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG55_RS24310 4635165..4636106 - 942 WP_001251313.1 ABC transporter permease -
RG55_RS24315 4636107..4637120 - 1014 WP_001542893.1 ABC transporter ATP-binding protein -
RG55_RS24320 4637138..4638283 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
RG55_RS24325 4638528..4639934 - 1407 WP_000760663.1 PLP-dependent aminotransferase family protein -
RG55_RS24330 4640013..4640429 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RG55_RS24335 4640475..4640651 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RG55_RS24340 4640873..4641103 + 231 WP_000494244.1 YncJ family protein -
RG55_RS24345 4641195..4643156 - 1962 WP_001442195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RG55_RS24350 4643229..4643765 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
RG55_RS24355 4643818..4645032 + 1215 WP_071609973.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4645072..4646220 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T51235 WP_000813794.1 NZ_CP010183:c4640651-4640475 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T51235 NZ_CP010183:c4640651-4640475 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT51235 WP_001270286.1 NZ_CP010183:c4640429-4640013 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT51235 NZ_CP010183:c4640429-4640013 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References