Detailed information of TA system TA051225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 4081316..4081787
• Replicon: chromosome
• Accession: NZ_CP010183
• Organism: Escherichia coli strain M3

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: -
• Locus tag: RG55_RS21270
• Protein ID: WP_061892358.1
• Coordinates: 4081316..4081594 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: RG55_RS21275
• Protein ID: WP_001302048.1
• Coordinates: 4081596..4081787 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG55_RS21225	4076752..4076955	-	204	WP_000096344.1	DUF4224 domain-containing protein	-
RG55_RS21230	4077014..4079455	-	2442	WP_073520620.1	exonuclease	-
RG55_RS21235	4079549..4079740	-	192	WP_001070255.1	DUF1482 family protein	-
RG55_RS21240	4079737..4079925	-	189	WP_000854558.1	cell division inhibition protein DicB	-
RG55_RS21250	4080493..4080711	-	219	WP_001171942.1	hypothetical protein	-
RG55_RS21260	4080871..4081026	-	156	WP_073520775.1	DUF1391 family protein	-
RG55_RS21270	4081316..4081594	-	279	WP_061892358.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG55_RS21275	4081596..4081787	-	192	WP_001302048.1	hypothetical protein	Antitoxin
RG55_RS21280	4081808..4082179	-	372	WP_135405263.1	hypothetical protein	-
RG55_RS21285	4082276..4082578	+	303	WP_000172735.1	transcriptional regulator	-
RG55_RS21290	4082575..4083000	+	426	WP_073520618.1	Rha family transcriptional regulator	-
RG55_RS21295	4083072..4084142	+	1071	WP_073520617.1	phage replisome organizer	-
RG55_RS21300	4084149..4084901	+	753	WP_073520616.1	ATP-binding protein	-
RG55_RS21305	4084924..4085637	+	714	WP_073520615.1	DUF1627 domain-containing protein	-
RG55_RS21310	4085653..4086075	+	423	WP_073520614.1	DUF977 family protein	-
RG55_RS21315	4086072..4086371	+	300	WP_001266136.1	DUF4406 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4074245..4121103	46858

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10647.22 Da
• Isoelectric Point: 5.5647

>T51225 WP_061892358.1 NZ_CP010183:c4081594-4081316 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDVDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T51225 NZ_CP010183:c4081594-4081316 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACGTAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCATTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTGGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT51225 WP_001302048.1 NZ_CP010183:c4081787-4081596 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT51225 NZ_CP010183:c4081787-4081596 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACAAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGGAAGGAACGGAGCAAGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CTP0