Detailed information of TA system TA051211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 28167..28702
• Replicon: plasmid A
• Accession: NZ_CP010181
• Organism: Escherichia coli strain M1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: RG54_RS26105
• Protein ID: WP_001270421.1
• Coordinates: 28415..28702 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: RG54_RS26100
• Protein ID: WP_001132895.1
• Coordinates: 28167..28418 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG54_RS26070	23721..24890	+	1170	WP_046201818.1	transposase	-
RG54_RS26075	24930..25103	+	174	Protein_27	DUF4113 domain-containing protein	-
RG54_RS26080	25244..26206	-	963	WP_046201819.1	ParB/RepB/Spo0J family partition protein	-
RG54_RS26085	26203..27408	-	1206	WP_000817642.1	AAA family ATPase	-
RG54_RS26095	27782..27994	+	213	WP_000402944.1	hypothetical protein	-
RG54_RS26100	28167..28418	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RG54_RS26105	28415..28702	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG54_RS26110	28716..28975	+	260	Protein_33	hypothetical protein	-
RG54_RS26115	28988..29278	+	291	WP_046201820.1	hypothetical protein	-
RG54_RS28190	29325..29692	+	368	Protein_35	DDE-type integrase/transposase/recombinase	-
RG54_RS26135	30336..31313	+	978	WP_000361612.1	RepB family plasmid replication initiator protein	-
RG54_RS26140	31592..32332	-	741	WP_046201823.1	tyrosine-type recombinase/integrase	-
RG54_RS26145	32453..32614	-	162	WP_064756529.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic	1..201930	201930
	-	inside	IScluster/Tn	-	-	19044..33878	14834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T51211 WP_001270421.1 NZ_CP010181:28415-28702 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T51211 NZ_CP010181:28415-28702 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTGATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT51211 WP_001132895.1 NZ_CP010181:28167-28418 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT51211 NZ_CP010181:28167-28418 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAACTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6