Detailed information of TA system TA051202

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2003997..2004832
• Replicon: chromosome
• Accession: NZ_CP010180
• Organism: Escherichia coli strain M1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: RG54_RS10585
• Protein ID: WP_073520673.1
• Coordinates: 2004455..2004832 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A085P7I7
• Locus tag: RG54_RS10580
• Protein ID: WP_001327592.1
• Coordinates: 2003997..2004365 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG54_RS10535	2000677..2001132	+	456	WP_000581502.1	hypothetical protein	-
RG54_RS10550	2001416..2002234	+	819	WP_001234639.1	DUF945 domain-containing protein	-
RG54_RS10560	2002573..2003052	+	480	WP_000706981.1	antirestriction protein	-
RG54_RS10565	2003068..2003544	+	477	WP_001186724.1	RadC family protein	-
RG54_RS10570	2003613..2003834	+	222	WP_000692300.1	DUF987 domain-containing protein	-
RG54_RS10580	2003997..2004365	+	369	WP_001327592.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG54_RS10585	2004455..2004832	+	378	WP_073520673.1	TA system toxin CbtA family protein	Toxin
RG54_RS28380	2004829..2005317	+	489	Protein_1904	hypothetical protein	-
RG54_RS10600	2005337..2005534	+	198	WP_000445282.1	DUF957 domain-containing protein	-
RG54_RS10605	2005619..2006461	+	843	WP_073520781.1	DUF4942 domain-containing protein	-
RG54_RS10610	2006742..2007278	-	537	WP_000942786.1	GspM family type II secretion system protein YghD	-
RG54_RS10615	2007280..2008458	-	1179	WP_032178690.1	type II secretion system protein GspL	-
RG54_RS10620	2008455..2009432	-	978	WP_000633238.1	type II secretion system minor pseudopilin GspK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC	1944613..2069245	124632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14317.23 Da
• Isoelectric Point: 8.5251

>T51202 WP_073520673.1 NZ_CP010180:2004455-2004832 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRF
STSPRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKR

Nucleotide

• Length: 378 bp

>T51202 NZ_CP010180:2004455-2004832 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCATCTCCAGTAACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTACGGCCTCACGCTAAATGACACACCGTTCTCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCCCGGTTC
AGCACTAGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13901.65 Da
• Isoelectric Point: 6.3165

>AT51202 WP_001327592.1 NZ_CP010180:2003997-2004365 [Escherichia coli]
VSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT51202 NZ_CP010180:2003997-2004365 [Escherichia coli]
GTGTCAGACACGCACCATGAGACAAATTATCCCGACGACTATAACAACCGCCCCTGGTGGGGGCTGCCCTGCACCATAAC
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGACTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A085P7I7