Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1419153..1419778 Replicon chromosome
Accession NZ_CP010180
Organism Escherichia coli strain M1

Toxin (Protein)


Gene name vapC Uniprot ID A0A2J1DF47
Locus tag RG54_RS07510 Protein ID WP_000911331.1
Coordinates 1419153..1419551 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag RG54_RS07515 Protein ID WP_000450524.1
Coordinates 1419551..1419778 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG54_RS07490 1415031..1415231 + 201 WP_000383836.1 YpfN family protein -
RG54_RS07495 1415341..1416039 - 699 WP_000679812.1 esterase -
RG54_RS07500 1416113..1418128 - 2016 WP_001543081.1 tRNA cytosine(34) acetyltransferase TmcA -
RG54_RS07505 1418143..1419006 - 864 WP_001267507.1 neutral zinc metallopeptidase -
RG54_RS07510 1419153..1419551 - 399 WP_000911331.1 tRNA(fMet)-specific endonuclease VapC Toxin
RG54_RS07515 1419551..1419778 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
RG54_RS07520 1419932..1420645 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
RG54_RS07530 1420858..1421892 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
RG54_RS07535 1421909..1422787 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
RG54_RS07540 1422933..1423505 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
RG54_RS07545 1423505..1423975 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14862.19 Da        Isoelectric Point: 9.2216

>T51199 WP_000911331.1 NZ_CP010180:c1419551-1419153 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLVELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T51199 NZ_CP010180:c1419551-1419153 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGGTGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT51199 WP_000450524.1 NZ_CP010180:c1419778-1419551 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT51199 NZ_CP010180:c1419778-1419551 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J1DF47

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References