Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 850350..850821 Replicon chromosome
Accession NZ_CP010180
Organism Escherichia coli strain M1

Toxin (Protein)


Gene name parE_1 Uniprot ID -
Locus tag RG54_RS04605 Protein ID WP_061892358.1
Coordinates 850543..850821 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag RG54_RS04600 Protein ID WP_001302048.1
Coordinates 850350..850541 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG54_RS04560 845766..846065 - 300 WP_001266136.1 DUF4406 domain-containing protein -
RG54_RS04565 846062..846484 - 423 WP_073520614.1 DUF977 family protein -
RG54_RS04570 846500..847213 - 714 WP_073520615.1 DUF1627 domain-containing protein -
RG54_RS04575 847236..847988 - 753 WP_073520616.1 ATP-binding protein -
RG54_RS04580 847995..849065 - 1071 WP_073520617.1 phage replisome organizer -
RG54_RS04585 849137..849562 - 426 WP_073520618.1 Rha family transcriptional regulator -
RG54_RS04590 849559..849861 - 303 WP_000172735.1 transcriptional regulator -
RG54_RS04595 849958..850329 + 372 WP_135405263.1 hypothetical protein -
RG54_RS04600 850350..850541 + 192 WP_001302048.1 hypothetical protein Antitoxin
RG54_RS04605 850543..850821 + 279 WP_061892358.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG54_RS04615 851111..851266 + 156 WP_073520775.1 DUF1391 family protein -
RG54_RS04625 851426..851644 + 219 WP_001171942.1 hypothetical protein -
RG54_RS04635 852212..852400 + 189 WP_000854558.1 cell division inhibition protein DicB -
RG54_RS04640 852397..852588 + 192 WP_001070255.1 DUF1482 family protein -
RG54_RS04645 852682..855123 + 2442 WP_073520620.1 exonuclease -
RG54_RS04650 855182..855385 + 204 WP_000096344.1 DUF4224 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 808173..870569 62396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10647.22 Da        Isoelectric Point: 5.5647

>T51197 WP_061892358.1 NZ_CP010180:850543-850821 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDVDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T51197 NZ_CP010180:850543-850821 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACGTAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCATTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTGGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT51197 WP_001302048.1 NZ_CP010180:850350-850541 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT51197 NZ_CP010180:850350-850541 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACAAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGGAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References