Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2408546..2409171 Replicon chromosome
Accession NZ_CP010178
Organism Escherichia coli strain H15

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag RG53_RS12445 Protein ID WP_000911329.1
Coordinates 2408546..2408944 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag RG53_RS12450 Protein ID WP_000450524.1
Coordinates 2408944..2409171 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG53_RS12425 2404453..2404653 + 201 WP_000383836.1 YpfN family protein -
RG53_RS12430 2404734..2405432 - 699 WP_000679812.1 esterase -
RG53_RS12435 2405506..2407521 - 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
RG53_RS12440 2407536..2408399 - 864 WP_001267495.1 neutral zinc metallopeptidase -
RG53_RS12445 2408546..2408944 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
RG53_RS12450 2408944..2409171 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
RG53_RS12455 2409326..2410039 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
RG53_RS12465 2410252..2411286 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
RG53_RS12470 2411303..2412181 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
RG53_RS12475 2412327..2412899 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
RG53_RS12480 2412899..2413369 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T51172 WP_000911329.1 NZ_CP010178:c2408944-2408546 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T51172 NZ_CP010178:c2408944-2408546 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT51172 WP_000450524.1 NZ_CP010178:c2409171-2408944 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT51172 NZ_CP010178:c2409171-2408944 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References