Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1449486..1450012 Replicon chromosome
Accession NZ_CP010178
Organism Escherichia coli strain H15

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag RG53_RS07410 Protein ID WP_000323025.1
Coordinates 1449486..1449773 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag RG53_RS07415 Protein ID WP_000534858.1
Coordinates 1449773..1450012 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG53_RS07375 1445168..1446127 - 960 WP_000592549.1 DUF523 and DUF1722 domain-containing protein -
RG53_RS07380 1446320..1446844 + 525 WP_000780584.1 lipocalin family protein -
RG53_RS07385 1447000..1447377 - 378 WP_001204787.1 antitermination protein -
RG53_RS07390 1447395..1448444 - 1050 WP_073527870.1 DUF968 domain-containing protein -
RG53_RS07395 1448446..1448724 - 279 WP_012304870.1 hypothetical protein -
RG53_RS07400 1448791..1449042 - 252 WP_000980994.1 hypothetical protein -
RG53_RS07405 1449259..1449414 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
RG53_RS07410 1449486..1449773 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
RG53_RS07415 1449773..1450012 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
RG53_RS07420 1450037..1450342 + 306 WP_001326990.1 hypothetical protein -
RG53_RS07430 1450545..1450877 + 333 WP_073527871.1 FlxA-like family protein -
RG53_RS07435 1451314..1452655 - 1342 Protein_1355 ISNCY family transposase -
RG53_RS26225 1453424..1453711 - 288 Protein_1356 hypothetical protein -
RG53_RS07460 1454322..1454678 - 357 WP_001374839.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1415875..1467190 51315


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T51162 WP_000323025.1 NZ_CP010178:c1449773-1449486 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T51162 NZ_CP010178:c1449773-1449486 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT51162 WP_000534858.1 NZ_CP010178:c1450012-1449773 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT51162 NZ_CP010178:c1450012-1449773 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References