Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3549364..3549995 Replicon chromosome
Accession NZ_CP010176
Organism Escherichia coli strain H10

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag RG51_RS18575 Protein ID WP_001259388.1
Coordinates 3549720..3549995 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag RG51_RS18570 Protein ID WP_000593555.1
Coordinates 3549364..3549723 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG51_RS18545 3545496..3546440 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
RG51_RS18550 3546437..3547270 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
RG51_RS18555 3547270..3548034 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
RG51_RS18560 3548031..3548837 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
RG51_RS18565 3548843..3549244 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
RG51_RS18570 3549364..3549723 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
RG51_RS18575 3549720..3549995 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
RG51_RS18580 3550068..3551192 - 1125 WP_001216257.1 ABC transporter permease -
RG51_RS18585 3551192..3553927 - 2736 WP_000149124.1 ribosome-associated ATPase/putative transporter RbbA -
RG51_RS18590 3553924..3554991 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T51128 WP_001259388.1 NZ_CP010176:c3549995-3549720 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T51128 NZ_CP010176:c3549995-3549720 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT51128 WP_000593555.1 NZ_CP010176:c3549723-3549364 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT51128 NZ_CP010176:c3549723-3549364 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References