Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 30222..30865 Replicon plasmid B
Accession NZ_CP010174
Organism Escherichia coli strain H8

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag RG50_RS26665 Protein ID WP_000754566.1
Coordinates 30449..30865 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag RG50_RS26660 Protein ID WP_001261276.1
Coordinates 30222..30452 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG50_RS26625 25341..25610 + 270 WP_000339857.1 hypothetical protein -
RG50_RS26630 25787..26653 - 867 WP_004118283.1 replication initiation protein -
RG50_RS28925 27183..27287 + 105 WP_032409716.1 hypothetical protein -
RG50_RS26635 27416..27673 + 258 WP_000764642.1 hypothetical protein -
RG50_RS26640 27731..28507 - 777 WP_000015958.1 site-specific integrase -
RG50_RS26645 28504..29247 - 744 WP_000129823.1 hypothetical protein -
RG50_RS26650 29298..29648 - 351 WP_000493378.1 hypothetical protein -
RG50_RS26660 30222..30452 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
RG50_RS26665 30449..30865 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
RG50_RS27970 31069..31925 + 857 Protein_35 IS3-like element ISEc15 family transposase -
RG50_RS26685 32207..33283 + 1077 WP_000227969.1 IS110 family transposase -
RG50_RS26695 33566..34426 + 861 WP_000557452.1 aminoglycoside N-acetyltransferase AAC(3)-IIe -
RG50_RS26700 34439..34981 + 543 WP_002063889.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul1 / qacE / aadA5 / dfrA17 / aac(3)-IIa / erm(B) / blaTEM-1B / blaCTX-M-15 - 1..106274 106274


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T51102 WP_000754566.1 NZ_CP010174:30449-30865 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T51102 NZ_CP010174:30449-30865 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT51102 WP_001261276.1 NZ_CP010174:30222-30452 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT51102 NZ_CP010174:30222-30452 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References