Detailed information of TA system TA051101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 69753..70354
• Replicon: plasmid A
• Accession: NZ_CP010173
• Organism: Escherichia coli strain H8

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: RG50_RS26340
• Protein ID: WP_001216045.1
• Coordinates: 69974..70354 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: RG50_RS26335
• Protein ID: WP_001190712.1
• Coordinates: 69753..69974 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG50_RS26285	64925..65812	+	888	WP_023153863.1	DUF551 domain-containing protein	-
RG50_RS26290	65895..66128	+	234	WP_000516537.1	hypothetical protein	-
RG50_RS26295	66307..66600	+	294	WP_063123741.1	hypothetical protein	-
RG50_RS26300	66607..66981	+	375	WP_000988658.1	hypothetical protein	-
RG50_RS26305	66963..67895	+	933	WP_000057449.1	hypothetical protein	-
RG50_RS26310	67892..68254	+	363	WP_001261544.1	hypothetical protein	-
RG50_RS29095	68329..68466	-	138	WP_000123562.1	hypothetical protein	-
RG50_RS26320	68916..69167	+	252	WP_001377386.1	DNA polymerase III subunit theta	-
RG50_RS26330	69291..69680	+	390	WP_000506726.1	DNA repair protein	-
RG50_RS26335	69753..69974	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RG50_RS26340	69974..70354	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
RG50_RS26345	70359..70538	+	180	WP_023153731.1	hypothetical protein	-
RG50_RS26350	70566..71609	+	1044	WP_000648825.1	DUF968 domain-containing protein	-
RG50_RS26355	71698..72150	+	453	WP_001369802.1	hypothetical protein	-
RG50_RS26360	72237..73430	+	1194	WP_073529428.1	terminase	-
RG50_RS26365	73430..74914	+	1485	WP_000124159.1	hypothetical protein	-
RG50_RS26370	75136..75255	+	120	WP_071527722.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94395	94395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T51101 WP_001216045.1 NZ_CP010173:69974-70354 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T51101 NZ_CP010173:69974-70354 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT51101 WP_001190712.1 NZ_CP010173:69753-69974 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT51101 NZ_CP010173:69753-69974 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6