Detailed information of TA system TA051091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4312074..4312712
• Replicon: chromosome
• Accession: NZ_CP010172
• Organism: Escherichia coli strain H8

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: RG50_RS22550
• Protein ID: WP_001447010.1
• Coordinates: 4312074..4312250 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: RG50_RS22555
• Protein ID: WP_001270286.1
• Coordinates: 4312296..4312712 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG50_RS22530	4307693..4308907	-	1215	WP_001326689.1	BenE family transporter YdcO	-
RG50_RS22535	4308960..4309496	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
RG50_RS22540	4309569..4311530	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
RG50_RS22545	4311622..4311852	-	231	WP_000494244.1	YncJ family protein	-
RG50_RS22550	4312074..4312250	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
RG50_RS22555	4312296..4312712	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
RG50_RS22560	4312791..4314197	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
RG50_RS22565	4314442..4315587	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
RG50_RS22570	4315605..4316618	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
RG50_RS22575	4316619..4317560	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T51091 WP_001447010.1 NZ_CP010172:4312074-4312250 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T51091 NZ_CP010172:4312074-4312250 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT51091 WP_001270286.1 NZ_CP010172:4312296-4312712 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT51091 NZ_CP010172:4312296-4312712 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8