Detailed information of TA system TA051089

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3123389..3124083
• Replicon: chromosome
• Accession: NZ_CP010172
• Organism: Escherichia coli strain H8

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: RG50_RS16270
• Protein ID: WP_001094398.1
• Coordinates: 3123715..3124083 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: RG50_RS16265
• Protein ID: WP_077249034.1
• Coordinates: 3123389..3123694 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG50_RS16235	3120122..3120355	+	234	WP_001117566.1	DUF905 family protein	-
RG50_RS16240	3120445..3121263	+	819	WP_001234397.1	DUF945 domain-containing protein	-
RG50_RS16245	3121355..3121840	+	486	WP_001586019.1	antirestriction protein	-
RG50_RS16250	3121856..3122332	+	477	WP_001424026.1	RadC family protein	-
RG50_RS16255	3122401..3122622	+	222	WP_000692345.1	DUF987 domain-containing protein	-
RG50_RS16260	3122641..3123285	+	645	WP_000086755.1	hypothetical protein	-
RG50_RS16265	3123389..3123694	+	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG50_RS16270	3123715..3124083	+	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
RG50_RS16275	3124404..3124742	-	339	Protein_2879	LysR family transcriptional regulator	-
RG50_RS16280	3124793..3125749	-	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
RG50_RS16285	3125759..3127957	-	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
RG50_RS16290	3127954..3128910	-	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T51089 WP_001094398.1 NZ_CP010172:3123715-3124083 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T51089 NZ_CP010172:3123715-3124083 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT51089 WP_077249034.1 NZ_CP010172:3123389-3123694 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT51089 NZ_CP010172:3123389-3123694 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4