Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1888391..1889189 Replicon chromosome
Accession NZ_CP010172
Organism Escherichia coli strain H8

Toxin (Protein)


Gene name yeeV Uniprot ID U9XMP3
Locus tag RG50_RS09940 Protein ID WP_000854735.1
Coordinates 1888391..1888768 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M1EW42
Locus tag RG50_RS09945 Protein ID WP_032153712.1
Coordinates 1888815..1889189 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG50_RS09900 1883617..1884546 + 930 Protein_1742 lipopolysaccharide biosynthesis protein -
RG50_RS09905 1884557..1885261 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
RG50_RS09910 1885301..1885621 - 321 Protein_1744 DUF4102 domain-containing protein -
RG50_RS09915 1886085..1886411 - 327 WP_000779483.1 type II toxin-antitoxin system RelE/ParE family toxin -
RG50_RS09920 1886408..1886671 - 264 WP_025492088.1 type II toxin-antitoxin system ParD family antitoxin -
RG50_RS09925 1886743..1887609 - 867 WP_032153714.1 DUF4942 domain-containing protein -
RG50_RS09930 1887694..1887891 - 198 WP_000839265.1 DUF957 domain-containing protein -
RG50_RS09935 1887903..1888394 - 492 WP_023155722.1 hypothetical protein -
RG50_RS09940 1888391..1888768 - 378 WP_000854735.1 TA system toxin CbtA family protein Toxin
RG50_RS09945 1888815..1889189 - 375 WP_032153712.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
RG50_RS09950 1889269..1889490 - 222 WP_000692345.1 DUF987 domain-containing protein -
RG50_RS09955 1889559..1890035 - 477 WP_001186715.1 RadC family protein -
RG50_RS09960 1890051..1890536 - 486 WP_032153711.1 antirestriction protein -
RG50_RS09965 1890628..1891446 - 819 WP_001234693.1 DUF945 domain-containing protein -
RG50_RS09970 1891537..1891746 - 210 WP_023155727.1 DUF905 family protein -
RG50_RS09975 1891776..1892453 - 678 WP_023155728.1 hypothetical protein -
RG50_RS09980 1892572..1893456 - 885 WP_024166630.1 50S ribosome-binding GTPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1884557..1885261 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14061.07 Da        Isoelectric Point: 8.2904

>T51084 WP_000854735.1 NZ_CP010172:c1888768-1888391 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T51084 NZ_CP010172:c1888768-1888391 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13697.51 Da        Isoelectric Point: 6.6240

>AT51084 WP_032153712.1 NZ_CP010172:c1889189-1888815 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT51084 NZ_CP010172:c1889189-1888815 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XMP3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1EW42

References