Detailed information of TA system TA051067

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1830744..1831423
• Replicon: chromosome
• Accession: NZ_CP010171
• Organism: Escherichia coli strain H7

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A8B3US07
• Locus tag: RG49_RS10140
• Protein ID: WP_007777710.1
• Coordinates: 1831082..1831423 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8B3USS8
• Locus tag: RG49_RS10135
• Protein ID: WP_007777712.1
• Coordinates: 1830744..1831061 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG49_RS10090	1826179..1827000	+	822	WP_053271797.1	DUF945 domain-containing protein	-
RG49_RS10095	1827217..1827918	+	702	WP_029039039.1	WYL domain-containing protein	-
RG49_RS10100	1827955..1828191	+	237	WP_001115854.1	hypothetical protein	-
RG49_RS10105	1828191..1828634	+	444	WP_029039038.1	hypothetical protein	-
RG49_RS10110	1828658..1829125	+	468	WP_029039037.1	hypothetical protein	-
RG49_RS10115	1829202..1829441	+	240	WP_029039036.1	DUF905 domain-containing protein	-
RG49_RS10120	1829543..1830001	+	459	WP_053271796.1	antirestriction protein	-
RG49_RS10125	1830017..1830493	+	477	WP_073477028.1	RadC family protein	-
RG49_RS10130	1830502..1830723	+	222	WP_053271794.1	DUF987 domain-containing protein	-
RG49_RS10135	1830744..1831061	+	318	WP_007777712.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG49_RS10140	1831082..1831423	+	342	WP_007777710.1	TA system toxin CbtA family protein	Toxin
RG49_RS10145	1832272..1833966	-	1695	WP_073477030.1	AAA family ATPase	-
RG49_RS10150	1834014..1834298	-	285	WP_130723338.1	hypothetical protein	-
RG49_RS10155	1834768..1835529	-	762	WP_073477032.1	hypothetical protein	-
RG49_RS10160	1835863..1836213	+	351	WP_000258782.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1805455..1873296	67841

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12934.88 Da
• Isoelectric Point: 8.0308

>T51067 WP_007777710.1 NZ_CP010171:1831082-1831423 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLQAVDILRARQATGLLQQSRSNVVR

Nucleotide

• Length: 342 bp

>T51067 NZ_CP010171:1831082-1831423 [Escherichia coli]
ATGAAAACTTTGCCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGGATCTCGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCGCCTTATCTTCAGGCTGTCGATATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCAGCAAAG
CCGTAGCAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12005.67 Da
• Isoelectric Point: 7.3723

>AT51067 WP_007777712.1 NZ_CP010171:1830744-1831061 [Escherichia coli]
MTHITWGLQRNITPRLGARLVQEGNRLHYLADRASITGKFSDTECRKLDETFPHFIRQMESMLTTGEVSPQHAHCVIMYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT51067 NZ_CP010171:1830744-1831061 [Escherichia coli]
ATGACTCACATCACATGGGGCCTGCAGCGGAATATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGAGGGGAACCGGTT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACACCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAAGTCAGCCCCCAACATGCCCACTGCGTCATCATGTACCAC
AACGGTTTCACCTGTGAAGCCGACACCCTTGGCAGTTGCGGTTACGTGTACATCGCCATTTATCCCACCCAGCGCTAA

Structures

Toxin

• AlphaFold DB: A0A8B3US07

Antitoxin

• AlphaFold DB: A0A8B3USS8