Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1611544..1612162 Replicon chromosome
Accession NZ_CP010171
Organism Escherichia coli strain H7

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag RG49_RS08805 Protein ID WP_001291435.1
Coordinates 1611944..1612162 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag RG49_RS08800 Protein ID WP_000344800.1
Coordinates 1611544..1611918 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG49_RS08790 1606633..1607826 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
RG49_RS08795 1607849..1610998 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
RG49_RS08800 1611544..1611918 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
RG49_RS08805 1611944..1612162 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
RG49_RS08810 1612334..1612885 + 552 WP_000102564.1 maltose O-acetyltransferase -
RG49_RS08815 1613001..1613471 + 471 WP_000136192.1 YlaC family protein -
RG49_RS08820 1613635..1615185 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
RG49_RS08825 1615227..1615496 - 270 Protein_1563 DUF1428 family protein -
RG49_RS08835 1615551..1616248 + 698 Protein_1564 IS1 family transposase -
RG49_RS08840 1616265..1616357 - 93 Protein_1565 DUF1428 family protein -
RG49_RS08850 1616736..1617047 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T51066 WP_001291435.1 NZ_CP010171:1611944-1612162 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T51066 NZ_CP010171:1611944-1612162 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT51066 WP_000344800.1 NZ_CP010171:1611544-1611918 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT51066 NZ_CP010171:1611544-1611918 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References