Detailed information of TA system TA051053

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3976818..3977497
• Replicon: chromosome
• Accession: NZ_CP010170
• Organism: Escherichia coli strain H6

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: RG48_RS20820
• Protein ID: WP_000854680.1
• Coordinates: 3977156..3977497 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: RG48_RS20815
• Protein ID: WP_000070396.1
• Coordinates: 3976818..3977135 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG48_RS20770	3971958..3972821	+	864	WP_032181192.1	GTPase family protein	-
RG48_RS20775	3972913..3973734	+	822	WP_049120334.1	DUF945 domain-containing protein	-
RG48_RS20780	3973951..3974652	+	702	Protein_3768	WYL domain-containing protein	-
RG48_RS20785	3974693..3974929	+	237	WP_045341362.1	hypothetical protein	-
RG48_RS20790	3974929..3975372	+	444	WP_000824224.1	hypothetical protein	-
RG48_RS25960	3975395..3975622	+	228	WP_075204105.1	hypothetical protein	-
RG48_RS20800	3975619..3976077	+	459	WP_000211838.1	antirestriction protein	-
RG48_RS20805	3976093..3976569	+	477	WP_000811693.1	RadC family protein	-
RG48_RS20810	3976578..3976799	+	222	WP_001568590.1	DUF987 domain-containing protein	-
RG48_RS20815	3976818..3977135	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
RG48_RS20820	3977156..3977497	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
RG48_RS20830	3978070..3979323	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
RG48_RS20835	3979335..3980438	-	1104	WP_001285288.1	glutamate 5-kinase	-
RG48_RS20840	3980726..3981781	+	1056	WP_000749881.1	phosphoporin PhoE	-
RG48_RS20845	3981820..3982221	-	402	WP_000174677.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagW/ecpD / yagV/ecpE / gmhA/lpcA	3889885..4001841	111956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T51053 WP_000854680.1 NZ_CP010170:3977156-3977497 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T51053 NZ_CP010170:3977156-3977497 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACGGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT51053 WP_000070396.1 NZ_CP010170:3976818-3977135 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT51053 NZ_CP010170:3976818-3977135 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7