Detailed information of TA system TA051026

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 2212375..2213054
• Replicon: chromosome
• Accession: NZ_CP010169
• Organism: Escherichia coli strain H5

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: RG47_RS11335
• Protein ID: WP_000854680.1
• Coordinates: 2212375..2212716 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: RG47_RS11340
• Protein ID: WP_000070396.1
• Coordinates: 2212737..2213054 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG47_RS11310	2207651..2208052	+	402	WP_000174677.1	sigma factor-binding protein Crl	-
RG47_RS11315	2208091..2209146	-	1056	WP_000749881.1	phosphoporin PhoE	-
RG47_RS11320	2209434..2210537	+	1104	WP_001285288.1	glutamate 5-kinase	-
RG47_RS11325	2210549..2211802	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
RG47_RS11335	2212375..2212716	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
RG47_RS11340	2212737..2213054	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
RG47_RS11345	2213073..2213294	-	222	WP_001568590.1	DUF987 domain-containing protein	-
RG47_RS11350	2213303..2213779	-	477	WP_000811693.1	RadC family protein	-
RG47_RS11355	2213795..2214253	-	459	WP_000211838.1	antirestriction protein	-
RG47_RS25780	2214250..2214477	-	228	WP_075204105.1	hypothetical protein	-
RG47_RS11365	2214500..2214943	-	444	WP_000824224.1	hypothetical protein	-
RG47_RS11370	2214943..2215179	-	237	WP_045341362.1	hypothetical protein	-
RG47_RS11375	2215220..2215921	-	702	Protein_2049	WYL domain-containing protein	-
RG47_RS11380	2216138..2216959	-	822	WP_049120334.1	DUF945 domain-containing protein	-
RG47_RS11385	2217051..2217914	-	864	WP_032181192.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	2188833..2280070	91237

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T51026 WP_000854680.1 NZ_CP010169:c2212716-2212375 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T51026 NZ_CP010169:c2212716-2212375 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACGGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT51026 WP_000070396.1 NZ_CP010169:c2213054-2212737 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT51026 NZ_CP010169:c2213054-2212737 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7