Detailed information of TA system TA051013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3775528..3776153
• Replicon: chromosome
• Accession: NZ_CP010167
• Organism: Escherichia coli strain H3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RG46_RS19530
• Protein ID: WP_000911330.1
• Coordinates: 3775528..3775926 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: RG46_RS19535
• Protein ID: WP_000450524.1
• Coordinates: 3775926..3776153 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG46_RS19510	3771435..3771635	+	201	WP_000383836.1	YpfN family protein	-
RG46_RS19515	3771716..3772414	-	699	WP_000679812.1	esterase	-
RG46_RS19520	3772488..3774503	-	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
RG46_RS19525	3774518..3775381	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
RG46_RS19530	3775528..3775926	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
RG46_RS19535	3775926..3776153	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
RG46_RS19540	3776307..3777020	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
RG46_RS19550	3777233..3778267	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
RG46_RS19555	3778284..3779162	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
RG46_RS19560	3779308..3779880	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
RG46_RS19565	3779880..3780350	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T51013 WP_000911330.1 NZ_CP010167:c3775926-3775528 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T51013 NZ_CP010167:c3775926-3775528 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT51013 WP_000450524.1 NZ_CP010167:c3776153-3775926 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT51013 NZ_CP010167:c3776153-3775926 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33