Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 21689..22211 Replicon plasmid A
Accession NZ_CP010164
Organism Escherichia coli strain H2

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag RG45_RS25020 Protein ID WP_000220560.1
Coordinates 21930..22211 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag RG45_RS25015 Protein ID WP_000121743.1
Coordinates 21689..21940 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG45_RS24985 17360..17641 + 282 WP_001185482.1 hypothetical protein -
RG45_RS24990 17666..18643 + 978 WP_024245156.1 hypothetical protein -
RG45_RS24995 18640..19065 - 426 WP_000202942.1 hypothetical protein -
RG45_RS25000 19133..19579 - 447 Protein_26 hypothetical protein -
RG45_RS25005 19619..20455 - 837 WP_001050931.1 replication initiation protein -
RG45_RS25015 21689..21940 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
RG45_RS25020 21930..22211 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG45_RS25040 22286..22983 + 698 Protein_30 IS1 family transposase -
RG45_RS25045 23285..23479 + 195 WP_053273080.1 hypothetical protein -
RG45_RS25050 23671..23922 - 252 Protein_32 IS200/IS605 family transposase -
RG45_RS26110 23978..24220 + 243 Protein_33 IS1 family transposase -
RG45_RS25070 24284..24988 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
RG45_RS25075 25041..25796 + 756 Protein_35 IS110-like element IS5075 family transposase -
RG45_RS25080 25978..26154 - 177 WP_000954592.1 Arm DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / aadA5 / sul2 / aph(3'')-Ib / aph(6)-Id - 1..29433 29433


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T50992 WP_000220560.1 NZ_CP010164:21930-22211 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T50992 NZ_CP010164:21930-22211 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT50992 WP_000121743.1 NZ_CP010164:21689-21940 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT50992 NZ_CP010164:21689-21940 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References