Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4346036..4346533 Replicon chromosome
Accession NZ_CP010163
Organism Escherichia coli strain H2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag RG45_RS23805 Protein ID WP_001173343.1
Coordinates 4346036..4346209 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag RG45_RS23810 Protein ID WP_000398619.1
Coordinates 4346237..4346533 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG45_RS23780 4341370..4342827 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
RG45_RS23785 4343064..4344581 + 1518 WP_001295074.1 lysine--tRNA ligase -
RG45_RS23800 4344753..4345981 + 1229 WP_088895425.1 IS3-like element IS2 family transposase -
RG45_RS23805 4346036..4346209 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
RG45_RS23810 4346237..4346533 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
RG45_RS23820 4346760..4347032 - 273 WP_000405647.1 N-acetyltransferase -
RG45_RS23825 4347044..4347274 - 231 WP_024250366.1 (4Fe-4S)-binding protein -
RG45_RS23830 4347455..4349086 + 1632 WP_001216477.1 sensor histidine kinase -
RG45_RS23835 4349083..4349802 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T50991 WP_001173343.1 NZ_CP010163:c4346209-4346036 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T50991 NZ_CP010163:c4346209-4346036 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT50991 WP_000398619.1 NZ_CP010163:c4346533-4346237 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT50991 NZ_CP010163:c4346533-4346237 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References