Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3618675..3619293 Replicon chromosome
Accession NZ_CP010163
Organism Escherichia coli strain H2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag RG45_RS19870 Protein ID WP_001291435.1
Coordinates 3619075..3619293 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag RG45_RS19865 Protein ID WP_000344800.1
Coordinates 3618675..3619049 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG45_RS19855 3613764..3614957 + 1194 WP_064506948.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
RG45_RS19860 3614980..3618129 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
RG45_RS19865 3618675..3619049 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
RG45_RS19870 3619075..3619293 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
RG45_RS19875 3619465..3620016 + 552 WP_000102564.1 maltose O-acetyltransferase -
RG45_RS19880 3620132..3620602 + 471 WP_000136192.1 YlaC family protein -
RG45_RS19885 3620766..3621158 + 393 Protein_3443 cyclic-guanylate-specific phosphodiesterase PdeB -
RG45_RS25960 3621174..3621872 - 699 Protein_3444 IS1 family transposase -
RG45_RS19905 3621928..3623094 + 1167 Protein_3445 cyclic-guanylate-specific phosphodiesterase PdeB -
RG45_RS19910 3623136..3623489 - 354 Protein_3446 DUF1428 family protein -
RG45_RS19920 3623868..3624179 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T50986 WP_001291435.1 NZ_CP010163:3619075-3619293 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T50986 NZ_CP010163:3619075-3619293 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT50986 WP_000344800.1 NZ_CP010163:3618675-3619049 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT50986 NZ_CP010163:3618675-3619049 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References