Detailed information of TA system TA050985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3230194..3230899
• Replicon: chromosome
• Accession: NZ_CP010163
• Organism: Escherichia coli strain H2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: RG45_RS17645
• Protein ID: WP_000539521.1
• Coordinates: 3230194..3230580 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RG45_RS17650
• Protein ID: WP_001280945.1
• Coordinates: 3230570..3230899 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG45_RS17625	3226198..3226824	+	627	WP_001295292.1	glutathione S-transferase GstB	-
RG45_RS17630	3226821..3227936	-	1116	Protein_3070	aldose sugar dehydrogenase YliI	-
RG45_RS17635	3228047..3228430	-	384	WP_000497137.1	biofilm formation regulator BssR	-
RG45_RS17640	3228643..3229968	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
RG45_RS17645	3230194..3230580	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG45_RS17650	3230570..3230899	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
RG45_RS17655	3230969..3232297	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
RG45_RS17660	3232305..3234653	-	2349	WP_001338403.1	EAL domain-containing protein	-
RG45_RS17665	3234831..3235742	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T50985 WP_000539521.1 NZ_CP010163:3230194-3230580 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T50985 NZ_CP010163:3230194-3230580 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT50985 WP_001280945.1 NZ_CP010163:3230570-3230899 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT50985 NZ_CP010163:3230570-3230899 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATAA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6