Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2603456..2604094 Replicon chromosome
Accession NZ_CP010163
Organism Escherichia coli strain H2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag RG45_RS14165 Protein ID WP_000813794.1
Coordinates 2603918..2604094 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RG45_RS14160 Protein ID WP_001270286.1
Coordinates 2603456..2603872 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG45_RS14140 2598608..2599549 - 942 WP_001251313.1 ABC transporter permease -
RG45_RS14145 2599550..2600563 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
RG45_RS14150 2600581..2601726 - 1146 WP_000047421.1 ABC transporter substrate-binding protein -
RG45_RS14155 2601971..2603377 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
RG45_RS14160 2603456..2603872 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RG45_RS14165 2603918..2604094 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RG45_RS14170 2604316..2604546 + 231 WP_000494244.1 YncJ family protein -
RG45_RS14175 2604638..2606599 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RG45_RS14180 2606672..2607208 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
RG45_RS14185 2607261..2608475 + 1215 WP_071607224.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T50984 WP_000813794.1 NZ_CP010163:c2604094-2603918 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T50984 NZ_CP010163:c2604094-2603918 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT50984 WP_001270286.1 NZ_CP010163:c2603872-2603456 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT50984 NZ_CP010163:c2603872-2603456 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References