Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1224981..1225564 Replicon chromosome
Accession NZ_CP010163
Organism Escherichia coli strain H2

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag RG45_RS06715 Protein ID WP_000254738.1
Coordinates 1225229..1225564 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1V2T5M9
Locus tag RG45_RS06710 Protein ID WP_000581941.1
Coordinates 1224981..1225229 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG45_RS06700 1221320..1222621 + 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
RG45_RS06705 1222669..1224903 + 2235 WP_000226815.1 GTP pyrophosphokinase -
RG45_RS06710 1224981..1225229 + 249 WP_000581941.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
RG45_RS06715 1225229..1225564 + 336 WP_000254738.1 endoribonuclease MazF Toxin
RG45_RS06720 1225635..1226426 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
RG45_RS06725 1226654..1228291 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
RG45_RS06730 1228379..1229677 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T50973 WP_000254738.1 NZ_CP010163:1225229-1225564 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T50973 NZ_CP010163:1225229-1225564 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9385.76 Da        Isoelectric Point: 4.4665

>AT50973 WP_000581941.1 NZ_CP010163:1224981-1225229 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT50973 NZ_CP010163:1224981-1225229 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2T5M9

References