Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1126207..1126861 Replicon chromosome
Accession NZ_CP010163
Organism Escherichia coli strain H2

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag RG45_RS06195 Protein ID WP_000244781.1
Coordinates 1126454..1126861 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG45_RS06190 Protein ID WP_000354046.1
Coordinates 1126207..1126473 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG45_RS06165 1121376..1122119 + 744 WP_032298494.1 SDR family oxidoreductase -
RG45_RS06170 1122176..1123609 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
RG45_RS06175 1123654..1123965 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
RG45_RS06180 1124129..1124788 + 660 WP_000250274.1 hemolysin III family protein -
RG45_RS06185 1124984..1125964 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
RG45_RS06190 1126207..1126473 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG45_RS06195 1126454..1126861 + 408 WP_000244781.1 protein YgfX Toxin
RG45_RS06200 1126901..1127422 - 522 WP_001055874.1 flavodoxin FldB -
RG45_RS06205 1127534..1128430 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG45_RS06210 1128455..1129165 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG45_RS06215 1129171..1130904 + 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T50972 WP_000244781.1 NZ_CP010163:1126454-1126861 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T50972 NZ_CP010163:1126454-1126861 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT50972 WP_000354046.1 NZ_CP010163:1126207-1126473 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT50972 NZ_CP010163:1126207-1126473 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References