Detailed information of TA system TA050963

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3425496..3426175
• Replicon: chromosome
• Accession: NZ_CP010160
• Organism: Escherichia coli strain H1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: RG44_RS18400
• Protein ID: WP_000854680.1
• Coordinates: 3425496..3425837 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: RG44_RS18405
• Protein ID: WP_000070396.1
• Coordinates: 3425858..3426175 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG44_RS18365	3421125..3422384	+	1260	WP_073544909.1	allantoate amidohydrolase	-
RG44_RS18370	3422417..3423394	-	978	WP_004179941.1	nitronate monooxygenase	-
RG44_RS18390	3423652..3424349	+	698	Protein_3220	IS1 family transposase	-
RG44_RS18395	3424365..3424790	-	426	Protein_3221	DDE-type integrase/transposase/recombinase	-
RG44_RS18400	3425496..3425837	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
RG44_RS18405	3425858..3426175	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
RG44_RS18410	3426194..3426415	-	222	WP_001568590.1	DUF987 domain-containing protein	-
RG44_RS18415	3426424..3426900	-	477	WP_000811693.1	RadC family protein	-
RG44_RS18420	3426916..3427374	-	459	WP_000211838.1	antirestriction protein	-
RG44_RS18425	3427472..3427711	-	240	WP_000194654.1	DUF905 domain-containing protein	-
RG44_RS18430	3427788..3428255	-	468	WP_001385283.1	hypothetical protein	-
RG44_RS18435	3428278..3428721	-	444	WP_000649865.1	hypothetical protein	-
RG44_RS18440	3428721..3428957	-	237	WP_001144031.1	hypothetical protein	-
RG44_RS18445	3428998..3429699	-	702	WP_000189411.1	WYL domain-containing protein	-
RG44_RS18450	3429916..3430737	-	822	WP_000197387.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3349225..3426175	76950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T50963 WP_000854680.1 NZ_CP010160:c3425837-3425496 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T50963 NZ_CP010160:c3425837-3425496 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT50963 WP_000070396.1 NZ_CP010160:c3426175-3425858 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT50963 NZ_CP010160:c3426175-3425858 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7